Calcium clamp in isolated neurones of the snail Helix pomatia.
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چکیده
منابع مشابه
Transmembrane outward hydrogen current in intracellularly perfused neurones of the snail Helix pomatia.
The ionic nature and pharmacological properties of the outward current activated by membrane depolarization were studied on isolated neurones of the snail Helix pomatia, placed in Na+- and Ca2+-free extracellular solutions and intracellularly perfused with K+-free solution ("nonspecific outward current"). It was shown that the amplitude and reversal potential of this current (estimated from ins...
متن کاملDynamic and static calcium gradients inside large snail (Helix aspersa) neurones detected with calcium-sensitive microelectrodes
We have used quartz Ca2+-sensitive microelectrodes (CASMs) in large voltage-clamped snail neurones to investigate the inward spread of Ca2+ after a brief depolarisation. Both steady state and [Ca2+]i transients changed with depth of penetration. When the CASM tip was within 20 microm of the far side of the cell the [Ca2+]i transient time to peak was 4.4+/-0.5s, rising to 14.7+/-0.7s at a distan...
متن کاملThe mobilization of calcium and bicarbonate by raised concentrations of potassium in the haemolymph of the snail, Helix pomatia.
1. After the concentration of potassium in the haemolymph of Helix pomatia has been raised by the infusion of KCl, it falls approximately exponentially for a time and then tends to rise. The accompanying rise and fall of calcium concentration can be fitted to a simple mathematical model. 2. The mobilization of calcium is accompanied by the generation of bicarbonate in equivalent amounts, withou...
متن کاملThe circulatory physiology of Helix pomatia. II. The isolated heart.
This work was undertaken to study the mechanical factors affecting the function of the isolated heart of Helix pomatia in order to help understand the variations in heart activity observed in intact animals. Biedermann (1884), working upon the isolated heart of Helix, observed that the frequency of beat increased with distension of the heart. Biering (1929) found that in the isolated Helix hear...
متن کاملThe modulatory effects of orexin B on the calcium channels activity in neuronal cells of Helix aspersa (garden snail)
Introduction: The functional effects of orexin-B on the calcium spikes and excitability of the neuronal soma membrane of garden snail, Helix aspersa were studied. Methods: Conventional intracellular recording, under the current clamp conditions was performed to examine the effects of orexin-B on the configuration and electrophysiological properties of calcium spikes. Results: Application o...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1993
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1993.sp019542